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12 Cards in this Set

  • Front
  • Back
Recognition of Ori Site
Prokaryote:
DNA A protein

Eukaryote:
unknown
Unwinding of double helix
Prokaryote:
Helicase (req's ATP)

Eukaryote:
Helicase (req's ATP)
Stabilization of unwound strands
Prokaryote:
SSB protein

Eukaryote:
SSB protein
Synthesis of RNA Primers
Prokaryote:
primase

Eukaryote:
primase
Synthesis of DNA (Leading & Lagging strands)
Prokaryote:
leading & lagging: DNA polymerase III

Eukaryote:
leading: DNA poly delta
lagging: DNA poly alpha
Removal of RNA primers
Prok:
DNA polymerase I (5'-->3' exonuclease) ...remember that the 3' exonuclease part of DNA poly III as a correction tool

Euk:
unknown
Replacement of RNA with DNA
Prok:
DNA poly I

Euk:
unknown
Joining of Okazaki Fragments
Prok:
DNA ligase I (req NAD)

Euk:
DNA ligase I (req ATP)
Removal of supercoils ahead of advancing replication forks
Prk:
DNA topoisomerase II (DNA gyrase)
[inh. by quinolones like nalidixic acid, ciprofloxacin, norlfoxacin]

Euk:
DNA topoisomerase II
[inh by anticancer: etoposide, teniposide]
Synthesis of telomeres
Prk: none

Euk: telomerase (adds to the end of the DNA b/c human DNA poly cuts off a bit with each replication)
DNA Damage:

Thymine Dimers (cause, recognition/excision enzyme, repair enzyme)
cause: UV radiation

cell cycle phase: G1

recognition/excision enzyme: Excision endonuclease-->deficient in Xeroderma Pigmentosum

repair enzyme: DNA poly, DNA ligase
DNA Damage:

Mismatched base (cause, recognition/excision enzyme, repair enzyme)
case: DNA replication error

cell cycle phase: G2

recognition/excisioin enzyme: mutation of hMSH2 or hMLH1 gene (recognition & repair genes) leads to HNPCC (lynch syndrome)

repair enzyme: DNA poly, DNA ligase