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17 Cards in this Set

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Enterotoxin
Chemical secreted by microorganisms 
that affects cells lining the intestinal tract
Coliform
Gram‐negative bacteria that ferment lactose to 
produce gas; found in the intestinal tracts of 
animals and humans
Lower temperatures have bacteriostatic effects on many microorganisms. Refrigeration can slow down, but not necessarily inhibit, microbial growth
How does refrigeration affect the microorganisms 
found in milk and food?
Microorganisms that can grow under anaerobic conditions, such as Clostridium botulinum, can
grow and thrive under vacuum sealed conditions and release toxins that cause food poisoning.
If improperly treated canned foods are vacuum 
sealed, what kind of bacteria might survive to  cause food poisoning?
Mannitol Salt agar (MSA)
The addition of high amounts of salts makes 
this medium highly  selective for the halophilic 
(salt‐preferring) organisms, such as the genus 
Staphylococcus;
Phenylethyl Alcohol agar (PEA)
Gram-negative organisms are partially inhibited 
by the addition of alcohol, making this medium 
selective for the growth of Gram‐positive organisms.
Mannitol Salt agar (MSA)
it is also differential for mannitol fermentation. Halophiles that ferment mannitol will appear yellow.
Phenylethyl Alcohol agar (PEA)
Streptococcus species such as Streptococcus enterococci produce small, pinpoint, white colonies.
Mannitol Salt agar (MSA)
For example, Staphylococcus aureus will ferment mannitol to produce yellow colonies surrounded by yellow zones, while Staphylococcus epidermidis will not ferment mannitol, growing as white colonies with no yellow zones.
Hektoen Enteric agar (HE)
A selective and differential medium designed to 
isolate and differentiate species of Salmonella and 
Shigella from other Enterobacteriaceae.
Salmonella‐Shigella agar (SSA)
Used to isolate Salmonella and Shigella, while 
coliforms are  inhibited.
Hektoen Enteric agar (HE)
Bile salts and the dyes bromthymol blue and acid fuchsin inihibit the growth of most Gram positive organisms.
Salmonella‐Shigella agar (SSA)
Species of the genus Salmonella will produce dark colonies.
Hektoen Enteric agar (HE)
Salmonella and Shigella species produce blueish/green colonies.
Salmonella‐Shigella agar (SSA)
Species of the genus Shigella are inhibited or produce green or brownish colonies.
Hektoen Enteric agar (HE)
Coliforms such as Escherichia coli produce salmon to orange colored colonies.
Bacteriological Examination Of Food
Micro Lab 12 - is