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27 Cards in this Set

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Divide gram positive bacteria into what divisions?

(note: there are exceptions)
Cocci and Bacilli
What is unique about Mycobacterium?
It is gram positive but doesn't belong in Cocci or bacilli.

Acid-fast.
What is unique about Actinomyces and Nocardia?
Gram positive but don't belong in either cocci and bacilli.

Branching filamentous growth (like fungi)
What kind of growth do Actinomycoes and Nocardia have? (it is like what organism?)
Branching filamentous growth (like fungi)
Gram + Cocci

Further division?
Catalase positive/negative

Positive: Staph
Negative: Strep
Gram + Cocci, Catalase positive

Further division?
Staph

Next decide whether it is coagulase Postive/negative
(+) S.aureus
(-) S.epidermis, S.saprophyticus
Gram+, Cocci, catalase positive, coagulase positive

What organism?
S.aureus
Gram+, Cocci, catalase positive, coagulase negative

What organisms?
S.epidermis
S.saprophyticus
Gram+, Cocci, catalase positive, coagulase negative

S.epidermis
S.saprophyticus

How do you differentiate b/w the two?
Novobiocin test.

Novobiocin sensitive: S.epidermis
Novobiocin resistance: S.saprophyticus
Gram+, Cocci, catalase positive, coagulase negative, Novobiocin sensitive
S.epidermis
Gram+, Cocci, catalase positive, coagulase negative, Novobiocin resistance
S.saphroyticus
Gram+, cocci, catalase negative
Strep
Gram+, cocci, catalase negative = Strep

How do you further divide the streps?
By thier hemolysis pattern (alpha, beta, random)
Gram+, cocci, catalase negative, Beta hemolytic

What organisms?
S.pyogenes
S.agalactiae (Group B strep)
Gram+, cocci, catalase negative Beta hemolytic

S.pyogenes
S.agalactiae

How do you differeniate b/w the two?
Bacitracin sensitive: S.pyogenes

Bacitracin resistant: S.agalactiae
Gram+, cocci, catalase negative, alpha hemolytic

What organism?
S.pneumoniae

S.mutans/S.intermedius
Gram+, cocci, catalase negative, alpha hemolytic

Optocin susceptible vs. resistant
Optocin susceptible: s.pneumoniae

Optocin resistant: S.mutans/S.intermedius
Gram+, cocci, catalase negative, alpha, beta, or gamma hemolytic

Group D strep (what organisms?)
E.faecalis
S.bovis
Gram+, cocci, catalase negative, alpha, beta, or gamma hemolytic

NaCl resistant or susceptible?
NaCl susceptible: S.bovis

NaCl resistant: E.faecalis
Gram+, bacilli

Further divide into?
Spore-forming vs. non spore-forming
Gram+, bacilli, spore forming
Bacillus
Clostridium
Gram+, bacilli, non-spore forming
Listeria
Corynebacterium
Gram+, bacilli, spore-forming

Aerobic vs. Obligate anaerobe?
Aerobic: bacillus

Obligate anaerobe: Clostridium
Gram+, bacilli, spore-forming, Aerobic

Bacillus

Motile vs. non-motile
Motile: B.cerus

Non-motile: B.anthracis
Gram+, bacilli, spore-forming, Anaerobe

Clostridium

Motile vs. non-motile
Motile: C.tetani, C.botulinum, C.difficle

Non-motile: C. perfringens
Gram+, bacilli, non spore-forming

Motile vs. non-motile
Motile: L.monocytogenes

Non-motile: C.diphtheraie
What is unique about Listeria (L.monocytogenes)
Only Gram positive which form Endotoxins