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56 Cards in this Set

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Just after decolorizer is applied acid-fast bacteria will appear ___ and non-acid bacteria will appear ______.

Fushcia and colorless

After brilliant green is applied as counter stain, acid-fast bacteria will appear _____ while non-acid bacteria appear _____.
Fuschia and green

Mycobacterium is acid-fast ___ (positive/negative) and ____ shaped

Positive


bacillus

Appearance of Staphylococcus aureus after acid-fast stain

Clusters of green cocci (acid-fast negative)

E. coli is gram ____ (negative/positive)

positive

Most critical step of the gram stain

Decolorization

If you forgot to counter stain at the end of a gram-stain procedure, gram negative would appear ______ and gram positive would appear _______

Colorless and purple

Microscopic morphology and Gram reaction for Staphylococcus aureus

gram-positive staphylococci

Why is it important crystal violet be contrasting color to safranin

Difference in color between the two stains allows you to differentiate between gram negative and gram positive cells by observing the color of organism

Microscopic morphology and Gram reaction for Micrococcus luteus

gram-positive staphylococci

Purpose of allowing bacteria smear to dry when placed on slide

Drying helps remove excess water to ensure optimal heat fixation and true arrangements are seen

Clusters of spherically shaped are called___

staphylococci

Chains of rod-shaped are called ____

Streptobacilli

Decolorizing agent in spore stain is _____

Water

Name two acid-fast organism.

Mycobacterium


Nocardia

______ bacteria retain carbol fuchsin after acid-alcohol, ______ are decolorized

Acid-fast


non-acid fast

The bacilli in this stain are gram _____

negative (pink)

These cocci are gram _____

These cocci are gram _____

positive (purple)

These bacteria are acid-fast _____

These bacteria are acid-fast _____

positive (fuchsia)

Do these bacteria contain endospores?

Yes, Endospore positive (pink with green)

These bacteria are acid-fast _____

negative (bluish-green)

Do these bacteria contain endospores?

Endospore negative (pink) no green endospores

1. The genus of this organism is ____


2. Describe the colonial morphology.

1. The genus of this organism is ____


2. Describe the colonial morphology.

Escherichia coli
beige, mucoid, glistening, opaque, medium convex, round, entire

1. The genus of this organism is ____


2. Describe the colonial morphology.

1. The genus of this organism is ____


2. Describe the colonial morphology.

Pseudomonas
Medium, opaque, circular, serrated, irredescent, beige with green pigment
Smells like grapes

1. The genus of this organism is ____


2. Describe the colonial morphology.

1. Serratia

2. Red, medium, irregular, circular, umbinate, opaque

1. The genus of this organism is ____


2. Describe the colonial morphology.

1. The genus of this organism is ____


2. Describe the colonial morphology.

1. Micrococcus
2. Bright buttery yellow, smaller, round, opaque, entire, convex, creamy

Fomite

Object or substance capable of carrying infectious organisms

Aseptically

Performed under sterile conditions
Density

Opaque, transparent, or translucent

Consistency

Butyrous, mucoid, dull, dry, or powdery

Texture
Smooth, rough, wrinkled, glistening, or irredescent
Serial dilution

Stepwise dilution of substance in solution

Colony forming unit- CFU

Viable bacteria; colony

TFTC
Too few to count
TNTC

Too numerous to count

Nosocomial infection

Infection development favored by a hospital environment

Gram stain reagents, time, and purpose

1. Crystal violet (primary stain)- 60 sec


2. Gram's iodine (mordant)-60 sec.


3. ethyl alcohol (decolorizer) 10-15 sec


4. safranin (counter stain) 30-60 sec

Acid-fast reagents, purpose, and time

1. Carbol fuchsin (primary stain) 5 min


2. acid-alcohol (decolorizer) 30 sec


3. brilliant green (counter stain) 2 min

Endospore stain reagents and time

1. Malachite green (endospore stain)-steam 5 min


2. safranin (vegetative stain)- 1 min

1. Name the organism we have seen in lab that could have been used to make this slide.


2. Describe the microscopic morphology


 

1. Name the organism we have seen in lab that could have been used to make this slide.


2. Describe the microscopic morphology


1. Bacillus


2. Gram positive streptobacilli

1. The genus of this organism is ____


2. Describe the colonial morphology.

1. The genus of this organism is ____


2. Describe the colonial morphology.

1. Mycobacterium


2. small, dry, pale yellow, rough

These bacteria are acid-fast _____

These bacteria are acid-fast _____

negative (blue-green)

1. The genus of this organism is ____
2. Describe the colonial morphology.

1. The genus of this organism is ____


2. Describe the colonial morphology.

1. Bacillus
2. Beige, dry, rough, opaque, large and flat

1. The genus of this organism is ____
2. Describe the colonial morphology.

1. The genus of this organism is ____


2. Describe the colonial morphology.

1. Staphylococcus aureus


2. medium, opaque, golden, round, entire, convex, smooth

1. Name the 3 organisms we have seen in lab that could have been used to make this slide.


2. Describe the microscopic morphology


 

1. Name the 3 organisms we have seen in lab that could have been used to make this slide.


2. Describe the microscopic morphology


1. Serratia, E. coli and Pseudomonas
2. Gram negative bacillus

1. Name the organism we have seen in lab that could have been used to make this slide.


2. Describe the microscopic morphology

1. Name the organisms we have seen in lab that could have been used to make this slide.


2. Describe the microscopic morphology

1. Staphylococcus aureus


Staphylococcus epidermidis


2. Gram positive staphylococci


Escherichia coli-


microscopic morphology _____
Gram _____
Acid-fast ____


Endospore _____

bacillus


gram negative


acid-fast negative


endospore negative

Staphylococcus aureus-


microscopic morphology ______
Gram _____
Acid-fast ____


Endospore _____

staphylococci


gram positive


acid-fast negative


endospore negative

Micrococcus luteus-


microscopic morphology _____
Gram _____
Acid-fast ____


Endospore _____

cocci in tetrads


gram positive


acid-fast negative


endospore negative

Bacillus subtilis-


microscopic morphology ______
Gram _____
Acid-fast ____


Endospore _____

streptobacilli


gram positive


acid-fast negative


endospore positive

Serratia-


microscopic morphology _____
Gram _____
Acid-fast ____


Endospore _____

bacillus


gram negative


acid-fast negative


endospore negative

Pseudomonas aerugenosa-


microscopic morphology ______
Gram _____
Acid-fast ____


Endospore _____

bacillus


gram negative


acid-fast negative


endospore negative

Mycobacterium-


microscopic morphology ______
Gram _____
Acid-fast ____


Endospore _____

bacillus


gram positive


acid-fast positive


endospore negative

1. Name the organism we have seen in lab that could have been used to make this slide.


2. Describe the microscopic morphology


 

1. Name the organism we have seen in lab that could have been used to make this slide.


2. Describe the microscopic morphology


1. Micrococcus


2. Gram positive tetrads

Staphylococcus epidermidis-


microscopic morphology ______


Gram _____


Acid-fast ____


Endospore _____

staphylococci


gram positive


acid-fast negative


endospore negative

1. The genus and species of this organism is ____
2. Describe the colonial morphology.

1. The genus and species of this organism is ____


2. Describe the colonial morphology.

1. Staphylococcus epidermidis


2. medium, round, opaque, flat, entire, white, creamy